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HX‐1171, a Novel Nrf2 Activator, Induces NQO1 and HMOX1 Expression
Author(s) -
Kim Jimin,
Shin SuHyun,
Ko YoungEun,
Miki Tokutaro,
Bae HeungMo,
Kang JongKoo,
Kim Jae Wha
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25993
Subject(s) - hmox1 , chemistry , activator (genetics) , transcription factor , microbiology and biotechnology , blot , enzyme , gclc , oxidative stress , cancer research , biochemistry , gene , glutathione , biology , heme , heme oxygenase
HX‐1171 (1‐O‐hexyl‐2,3,5‐trimethylhydroquinone) is a novel synthesized vitamin E derivative, which reportedly has positive effects on various diseases and conditions, such as liver fibrosis, hepatic cirrhosis, and cancer. In this study, we analyzed the transcriptional activity induced by HX‐1171. Results from reverse transcription polymerase chain reaction and promoter assays reveal that HX‐1171 increased the expression of NQO1 and HMOX1 , encoding antioxidant‐related enzymes, in A549 human lung epithelial cells. The activity of nuclear factor‐E2‐related factor (Nrf2), a key transcriptional factor for antioxidative enzymes, was examined in HX‐1171‐treated cells. Confocal microscopy and Western blotting showed that HX‐1171 effectively induced the nuclear translocation and transcriptional activity of Nrf2. We conclude that HX‐1171, a novel Nrf2 activator, may be a promising therapeutic agent for oxidative stress‐induced diseases. J. Cell. Biochem. 118: 3372–3380, 2017. © 2017 Wiley Periodicals, Inc.