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Effects Of Hypoxia in Long‐Term In Vitro Expansion of Human Bone Marrow Derived Mesenchymal Stem Cells
Author(s) -
Pezzi Annelise,
Amorin Bruna,
Laureano Álvaro,
Valim Vanessa,
Dahmer Alice,
Zambonato Bruna,
Sehn Filipe,
Wilke Ianaê,
Bruschi Lia,
Silva Maria Aparecida Lima da,
FilippiChiela Eduardo,
Silla Lucia
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25953
Subject(s) - mesenchymal stem cell , oxygen tension , microbiology and biotechnology , stem cell , hypoxia (environmental) , haematopoiesis , biology , stromal cell , oxidative stress , bone marrow , immunophenotyping , chemistry , immunology , flow cytometry , cancer research , biochemistry , oxygen , organic chemistry
Mesenchymal stem cells (MSC) are considered multipotent stromal, non‐hematopoietic cells with properties of self‐renovation and differentiation. Optimal conditions for culture of MSC have been under investigation. The oxygen tension used for cultivation has been studied and appears to play an important role in biological behavior of mesenchymal cells. The aim is characterize MSC in hypoxia and normoxia conditions comparing their morphological and functional characteristics. Bone marrow‐derived mesenchymal stem cells obtained from 15 healthy donors and cultured. MSC obtained from each donor were separated into two cultivation conditions normoxia (21% O 2 ) and hypoxia (three donors at 1%, three donors at 2%, five donors at 3%, and four donors at 4% O 2 ) up to second passage. MSC were evaluated for proliferation, differentiation, immunophenotyping, size and cell complexity, oxidative stress, mitochondrial activity, and autophagy. Culture conditions applied did not seem to affect immunophenotypic features and cellular plasticity. However, cells subjected to hypoxia showed smaller size and greater cellular complexity, besides lower proliferation ( P < 0.002). Furthermore, cells cultured in low O 2 tension had lower mitochondrial activity ( P < 0.03) and a reduced tendency to autophagy, although oxidative stress did not vary among groups ( P < 0.39). Oxygen tension seems to be a key regulator of cellular adaptation in vitro, and metabolic effects underlying this variable remain undescribed. Heterogeneity or even lack of results on the impact of oxygen concentration used for expanding MSC highlights the need for further research, in order to optimize conditions of cultivation and expansion and achieve greater safety and therapeutic efficacy. J. Cell. Biochem. 118: 3072–3079, 2017. © 2017 Wiley Periodicals, Inc.