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Resveratrol Enhances Self‐Renewal of Mouse Embryonic Stem Cells
Author(s) -
Li Na,
Du Zhaoyu,
Shen Qiaoyan,
Lei Qijing,
Zhang Ying,
Zhang Mengfei,
Hua Jinlian
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25942
Subject(s) - embryonic stem cell , microbiology and biotechnology , induced pluripotent stem cell , stem cell , biology , cellular differentiation , resveratrol , stat3 , embryoid body , signal transduction , genetics , biochemistry , gene
Resveratrol (RSV) has been shown to affect the differentiation of several types of stem cells, while the detailed mechanism is elusive. Here, we aim to investigate the function of RSV in self‐renewal of mouse embryonic stem cells (ESCs) and the related mechanisms. In contrast with its reported roles, we found unexpectedly that differentiated ESCs or iPSCs treated by RSV would not show further differentiation, but regained a naïve pluripotency state with higher expressions of core transcriptional factors and with the ability to differentiate into all three germ layers when transplanted in vivo. In accordance with these findings, RSV also enhanced cell cycle progression of ESCs via regulating cell cycle‐related proteins. Finally, enhanced activation of JAK/STAT3 signaling pathway and suppressed activation of mTOR were found essential in enhancing the self‐renewal of ESCs by RSV. Our finding discovered a novel function of RSV in enhancing the self‐renewal of ESCs, and suggested that the timing of treatment and concentration of RSV determined the final effect of it. Our work may contribute to understanding of RSV in the self‐renewal maintenance of pluripotent stem cells, and may also provide help to the generation and maintenance of iPSCs in vitro. J. Cell. Biochem. 118: 1928–1935, 2017. © 2017 Wiley Periodicals, Inc.