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MicroRNA‐184 Modulates Human Central Nervous System Lymphoma Cells Growth and Invasion by Targeting iASPP
Author(s) -
Liang Xiaogong,
Meng Wentong,
Hu Lianjie,
Li Lin,
Xing Hongyun,
Xie Gan,
Wang Anqiong,
Jia Yongqian
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25856
Subject(s) - microrna , small hairpin rna , cell growth , cancer research , biology , pi3k/akt/mtor pathway , protein kinase b , three prime untranslated region , suppressor , cell , untranslated region , signal transduction , cell culture , microbiology and biotechnology , messenger rna , cancer , gene knockdown , gene , genetics
Central nervous system lymphoma (CNSL) remains a diagnostical and therapeutical challenge. MiRNAs post‐transcriptionally regulate expression of targeted mRNAs through binding to their 3′ UTR to inhibit their translation or promote their degradation. Oncoprotein inhibitory member of the ASPP family (iASPP), a key inhibitor of tumor suppressor p53, has been reported to play oncogenic role in cancers. Our present study was aimed to determine whether the miR‐184/iASPP axis is involved in the proliferation and invasion of CNSL. A reduced level of miR‐184 was observed in CNSL tissues. Exogenous miR‐184 inhibited cell survival and invasion, as well as the tumor volumes, while miR‐184 inhibition could reverse this process. The RNA and protein levels of iASPP were significantly inhibited by miR‐184, and the 3′ UTR of iASPP was shown to be a target of miR‐184. The expression of iASPP was up‐regulated in CNSL tissues, compared to that of the normal brain tissues. The inhibition of iASPP by shRNA iASPP significantly repressed CNSL cells’ proliferation and invasion, and reduced the volume of the tumor. Besides, iASPP overexpression could partly restore the suppressive effect of miR‐184 on CNSL cell proliferation and invasive capability. We also revealed that miR‐184/iASPP axis regulated the proliferation and invasion via PI3K/Akt signaling pathway, which presents a novel potential therapy for intervention of CNSL. Taken together, our findings revealed the detailed role of the miR‐184/iASPP axis in CNSL and this axis might modulate the proliferation and invasion of CNSL via regulating the PI3K/Akt signaling pathway. J. Cell. Biochem. 118: 2645–2653, 2017. © 2017 Wiley Periodicals, Inc.