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Protein Expression Differences of 2‐Dimensional and Progressive 3‐Dimensional Cell Cultures of Non‐Small‐Cell‐Lung‐Cancer Cell Line H460
Author(s) -
Ravi Maddaly,
Mohan Divya K.,
Sahu Bellona
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25800
Subject(s) - cell culture , in vivo , cell , in vitro , lung cancer , biology , microbiology and biotechnology , 3d cell culture , cancer , cancer research , pathology , medicine , biochemistry , genetics
Non‐small‐cell‐lung‐cancer (NSCLC) constitutes about 75–80% of lung cancers. The challenge to tackle cancers is in early diagnosis and arriving at safer therapeutic options. In vitro studies using cancer cell lines continue to contribute significantly in understanding cancers. Cell culture methods have evolved and the recent developments in 3 dimensional (3D) cell cultures are inducing greater resemblance of the in vitro cultured cells with in vivo conditions. In this study, we established 3D aggregates of H460 cell line on agarose hydrogels and studied the protein expression differences among cells grown as monolayers (2D) and the progressively developing 3D aggregates from days 2 to 10. Analysis included matching of those proteins expressed by the developing aggregates and the available literature on progressing tumors in vivo. J. Cell. Biochem. 118: 1648–1652, 2017. © 2016 Wiley Periodicals, Inc.