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Bacterial and Arachnid Sphingomyelinases D: Comparison of Biophysical and Pathological Activities
Author(s) -
Mariutti Ricardo Barros,
ChavesMoreira Daniele,
Vuitika Larissa,
Caruso Ícaro Putinhon,
Coronado Monika A.,
Azevedo Vasco A.,
Murakami Mario T.,
Veiga Silvio Sanches,
Arni Raghuvir K.
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25781
Subject(s) - sphingomyelin , biology , enzyme , photobacterium phosphoreum , photobacterium , sphingomyelin phosphodiesterase , biochemistry , microbiology and biotechnology , vibrio , bacteria , genetics , membrane
Sphingomyelinases D have only been identified in arachnid venoms, Corynebacteria , Arcanobacterium , Photobacterium and in the fungi Aspergillus and Coccidioides . The arachnid and bacterial enzymes share very low sequence identity and do not contain the HKD sequence motif characteristic of the phospholipase D superfamily, however, molecular modeling and circular dichroism of SMases D from Loxosceles intermedia and Corynebacterium pseudotuberculosis indicate similar folds. The phospholipase, hemolytic and necrotic activities and mice vessel permeabilities were compared and both enzymes possess the ability to hydrolyze phospholipids and also promote similar pathological reactions in the host suggesting the existence of a common underlying mechanism in tissue disruption. J. Cell. Biochem. 118:2053–2063, 2017. © 2016 Wiley Periodicals, Inc.