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Does the Harvesting Technique Affect the Properties of Adipose‐Derived Stem Cells?—The Comparative Biological Characterization
Author(s) -
Bajek Anna,
Gurtowska Natalia,
Olkowska Joanna,
Maj Małgorzata,
Kaźmierski Łukasz,
Bodnar Magdalena,
Marszałek Andrzej,
Dębski Robert,
Drewa Tomasz
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25724
Subject(s) - characterization (materials science) , adipose tissue , stem cell , affect (linguistics) , chemistry , microbiology and biotechnology , biology , materials science , nanotechnology , biochemistry , psychology , communication
The objective of this study was to evaluate complex biological properties of human stem cells isolated from adipose tissue (ASCs) harvested utilizing different methods: surgical resection (R), power‐assisted liposuction (PAL), and laser‐assisted liposuction (LAL). ASCs were isolated from healthy donors, due to surgical resection, power‐, and laser‐assisted liposuction. Isolated cells were characterized by their clonogenicity, proliferation rate, doubling time, multilineage differentiation, and senescence potential. The average number of ASCs from 1g/1 ml of solid adipose tissue/lipoaspirate was 2.9 × 10 5 ± 2.4 × 10 5 , 1.1 × 10 5 ± 0.8 × 10 5 , and 1.2 × 10 5 ± 0.7 × 10 5 , respectively, for ASCsR, ASCsPAL, and ASCsLAL. However, number of colonies formed by ASCsR and ASCsPAL was significantly higher compared to the average number of colonies formed by ASCsLAL. Also, in comparison to other analyzed cell groups, ASCsPAL obtained the highest proliferative activity. All analyzed cells were characterized by stable expression of CD90 and CD44 markers during prolonged culture. Expression of CD34 and CD45 markers was decreasing in subsequent passages. Presented study shows that different ASCs collection method affects some basic characteristics of these cells, such as number of isolated cells, clonogeneity, or doubling time. J. Cell. Biochem. 118: 1097–1107, 2017. © 2016 Wiley Periodicals, Inc.