Premium
Anti‐Inflammatory and Anti‐Oxidant Effects of p‐Chloro‐phenyl‐selenoesterol on TNBS‐Induced Inflammatory Bowel Disease in Mice
Author(s) -
Zarzecki Micheli Stéfani,
Bortolotto Vandreza Cardoso,
Poetini Márcia Rósula,
Araujo Stífani Machado,
de Paula Mariane Trindade,
Roman Silvane Souza,
Spiazzi Cristiano,
Cibin Franciele Weber Santos,
Rodrigues Oscar Endrigo Dorneles,
Jesse Cristiano Ricardo,
Prigol Marina
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25670
Subject(s) - myeloperoxidase , colitis , inflammatory bowel disease , superoxide dismutase , proinflammatory cytokine , thiobarbituric acid , oxidative stress , tumor necrosis factor alpha , catalase , antioxidant , inflammation , pharmacology , chemistry , immunology , reactive oxygen species , medicine , biochemistry , lipid peroxidation , disease
This study aims to investigate the protective effect of p‐chloro‐phenyl‐selenoesterol [PCS; 0,2 mg/kg; 10 ml/kg i.g.) in colitis induced by 2,4,6‐trinitrobenzene sulfonic acid [TNBS; 2 mg/100 µl 50% ethanol; intrarectally) in mice. Several parameters including weight, length, histological analyses determination, thiobarbituric acid reactive species, reactive species levels, superoxide dismutase, catalase, and myeloperoxidase (MPO) activity of colon were evaluated. The serum levels of tumor necrosis factor alpha [TNF‐α) and interleukin 6 [IL‐6) were also assessed. Treatment with PCS reduced the clinical and histopathologic severity of TNBS‐induced colitis, characterized by colon length reduction and increased colon weight and microscopic intestinal inflammation. The therapeutic effects of PCS in this model were associated with significant decrease in proinflammatory cytokines TNF‐α and IL‐6 and decrease in MPO activity. Furthermore, combined with improvements in inflammatory parameters, treatment with the PCS was able to decrease oxidative stress and to prevent the decrease in antioxidant defenses in animals with TNBS‐induced colitis. This finding suggests that PCS can improve experimental colitis in mice and it could be a potential therapeutic agent for the treatment of patients with IBD. J. Cell. Biochem. 118: 709–717, 2017. © 2016 Wiley Periodicals, Inc.