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Diphenyl Diselenide Reduces Oxidative Stress and Toxicity Caused by HSV‐2 Infection in Mice
Author(s) -
Sartori Gláubia,
Jardim Natália Silva,
Sari Marcel Henrique Marcondes,
Flores Eduardo F.,
Prigol Marina,
Nogueira Cristina W.
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25667
Subject(s) - diphenyl diselenide , oxidative stress , superoxide dismutase , chemistry , toxicity , malondialdehyde , kidney , pharmacology , antioxidant , catalase , medicine , biochemistry , biology , selenium , organic chemistry
Herpes simplex viruses can cause uncommon systemic complications as acute liver failure (ALT) or urinary tract dysfunctions. Diphenyl diselenide, (PhSe) 2 , a classical studied organic selenium compound, has a novel antiviral action against HSV‐2 infection and well‐known antioxidant and anti‐inflammatory properties. This study aimed to investigate if (PhSe) 2 reduces oxidative stress and systemic toxicity caused by HSV‐2 infection in mice. Adult BALB/c mice were pre‐treated with (PhSe) 2 (5 mg kg −1 /day, intragastric, i.g.) during 5 days; at day 6 mice were infected with HSV‐2 (10 μl‐10 5 PFU/mL −1 ) and post‐treated with (PhSe) 2 for more 5 days. At day 11, they were killed and samples of liver and kidney were obtained to determine: reactive species (RS); malondialdehyde (MDA), and non‐protein thiols (NPSH) levels; the activities of antioxidant enzymes, superoxide dismutase (SOD), and catalase (CAT). The activities of adenosine deaminase (ADA), Na + /K + ‐ATPase (liver and kidney); alanine aminotransferase (ALT), aspartate aminotransferase (AST), and the levels of urea (plasma) were determined as markers of hepatic and renal toxicity. The results revealed that (PhSe) 2 treatment was effective against the increase of renal and hepatic oxidative stress in infected mice and also normalized hepatic and renal ADA activity. It recovered the activity of Na + /K + ‐ and was not effective against the increase in urea levels in infected mice. Different from (PhSe) 2 , acyclovir (positive control), caused an increase in ADA activity and a decrease in hepatic CAT activity. Considering the interest of alternative therapies to treat HSV‐2 infections and secondary complications, (PhSe) 2 become a notable candidate. J. Cell. Biochem. 118: 1028–1037, 2017. © 2016 Wiley Periodicals, Inc.