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Glucose Promotes a Pro‐Oxidant and Pro‐Inflammatory Stromal Microenvironment Which Favors Motile Properties in Breast Tumor Cells
Author(s) -
Kallens Violeta,
Tobar Nicolás,
Molina Jessica,
Bidegain Arantzazú,
Smith Patricio C.,
Porras Omar,
Martínez Jorge
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25650
Subject(s) - stromal cell , tumor microenvironment , cancer research , microbiology and biotechnology , chemistry , inflammation , biology , medicine , tumor cells
Chronic inflammation and metabolic reprogramming have been proposed as hallmarks of cancer development. Currently, many of the functional clues between these two phenomena are studied under the integrative view of functional stroma–epithelia interaction. It has been proposed that stromal cells, due to their abundance and avidity for glucose, are able to modify the metabolic behavior of an entire solid tumor. In the present study, using a mammary stromal cell line derived from healthy tissue subjected to long‐term culture in low (5 mM) or high (25 mM) glucose, we found that the hyperglycemic condition favors the establishment of a pro‐inflammatory and pro‐oxidant environment characterized by the induction of the COX‐2/PGE2 axis. In this condition, epithelial migration was stimulated. Moreover, we also found that stromal‐derived PGE2, acting as a stimulator of IL‐1 epithelial expression was one of the factors that promote the acquisition of motile properties by epithelial cells and the maintenance of a COX‐2/PGE2‐dependent inflammatory condition. Overall, our work provides experimental evidence that glucose stimulates a tumor inflammatory environment that, as a result of a functional cross‐talk between stroma and epithelia, may be responsible for tumor progression. J. Cell. Biochem. 118: 994–1002, 2017. © 2016 Wiley Periodicals, Inc.