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Effects of Environmental Pollutants on MCF‐7 Cells: A Metabolic Approach
Author(s) -
Norberto Sónia,
Calhau Conceição,
Pestana Diogo,
Faria Ana
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25645
Subject(s) - pollutant , pesticide , carbohydrate metabolism , metabolism , chemistry , glycolysis , mcf 7 , cell , toxicity , xenobiotic , enzyme , biochemistry , environmental chemistry , cancer cell , biology , cancer , ecology , genetics , human breast , organic chemistry
Several environmental pollutants (EPs) have been associated with biological and molecular processes leading to adverse human health effects, including different types of cancer. Nevertheless, the effects exerted on tumor glucose metabolism are unclear. To evaluate the effects on cellular and molecular mechanisms, namely glucose metabolism, MCF‐7 cells were exposed to EPs during short‐ and long‐term exposures. The effect of both, organochlorine pesticides and plasticizing agents, on glucose uptake by MCF‐7 cells was not dose‐dependent and was affected by time of exposure. The ΣHCH and BPA increased glucose uptake after 20 min. Long‐term exposure to 250 nM of organochlorine pesticides ( p , p′ ‐DDE and ΣHCH) and BPA increased cell proliferation. However, only the organochlorine pesticides were able to increase lactate production, without a concomitant higher glucose uptake or glycolytic enzymes transcription. Given their distinct persistent profiles, the biological significance of their exposure should be considered accordingly. J. Cell. Biochem. 118: 366–375, 2017. © 2016 Wiley Periodicals, Inc.