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Curcumin Promotes Osteosarcoma Cell Death by Activating miR‐125a/ERRα Signal Pathway
Author(s) -
Chen Peng,
Wang Haibin,
Yang Fan,
Chen Hongwu,
He Wei,
Wang Junjian
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25612
Subject(s) - curcumin , osteosarcoma , apoptosis , cancer research , downregulation and upregulation , programmed cell death , cell growth , chemistry , gene silencing , cell , signal transduction , small interfering rna , microbiology and biotechnology , biology , rna , biochemistry , gene
Curcumin has demonstrated valuable therapeutic potential against a variety of human cancers including osteosarcoma. However, the molecular mechanisms underlying its anti‐tumor effect remain to be poorly understood. By RNA sequence profiling, we found that curcumin significantly down‐regulates the expression of estrogen‐related receptor alpha (ERRα) in osteosarcoma cells. Overexpression of ERRα diminished curcumin‐activated apoptotic cell death and scavenged curcumin‐induced reactive oxygen species (ROS), while ERRα silencing sensitized osteosarcoma cells to curcumin, resulting in increased inhibition of cell proliferation. In addition, we found that curcumin suppressed the ERRα gene expression through upregulation of miR‐125a. Data from this study revealed a novel mechanism for curcumin‐mediated apoptotic cell death, which involves tumor cell killing via activating miR‐125a/ERRα pathway. Our studies also provide further support for osteosarcoma therapy by targeting ERRα alone or in combination with curcumin. J. Cell. Biochem. 118: 74–81, 2017. © 2016 Wiley Periodicals, Inc.