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Use of α v Integrin Linked to GFP to Image Molecular Dynamics in Trafficking Cancer‐Cell Emboli
Author(s) -
Tome Yasunori,
Yano Shuya,
Sugimoto Naotoshi,
Mii Sumiyuki,
Bouvet Michael,
Tsuchiya Hiroyuki,
Kanaya Fuminori,
Hoffman Robert M.
Publication year - 2017
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25603
Subject(s) - green fluorescent protein , pathology , osteosarcoma , integrin , metastasis , chemistry , in vivo , in vitro , embolus , cancer cell , cell , cancer , medicine , biology , surgery , gene , biochemistry , microbiology and biotechnology
Human osteosarcoma cells with an α v integrin green fluorescent protein (GFP) fusion gene were previously established and imaged in vitro and in vivo. In the present study, we imaged the interaction of α v integrin‐GFP in osteosarcoma cells and collagen fibers in vascular‐trafficking osteosarcoma emboli in nude mice. Human 143B osteosarcoma cells, expressing α v integrin‐GFP, were injected by a vascular route in an abdominal skin flap in nude mice. Osteosarcoma cells were fluorescently imaged in the epigastric cranialis vein in the abdominal skin flap by confocal microscopy. Collagen fibers were imaged in reflectance mode. At early stages of tumor embolus‐formation, cancer cells adhered firmly to each other, diffusely expressing α v integrin‐GFP. Two weeks after injection, collagen fiber scaffolds were visualized at the margins of tumor emboli or within them. Four weeks after injection, cancer cells invading from emboli were strongly expressing α v integrin‐GFP, and were aligned along collagen fibers. The results suggest α v integrin and collagen fiber scaffolds are important for tumor embolus formation, which are potential seeds of metastasis. J. Cell. Biochem. 118: 26–30, 2017. © 2016 Wiley Periodicals, Inc.