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HMGB1 Osteo‐Modulatory Action on Osteosarcoma SaOS‐2 Cell Line: An Integrated Study From Biochemical and ‐Omics Approaches
Author(s) -
Martinotti Simona,
Patrone Mauro,
Manfredi Marcello,
Gosetti Fabio,
Pedrazzi Marco,
Marengo Emilio,
Ranzato Elia
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25549
Subject(s) - hmgb1 , microbiology and biotechnology , cell , osteoblast , cell growth , cell culture , in vitro , chemistry , cell migration , wound healing , cancer research , biology , biochemistry , immunology , receptor , genetics
High mobility group box protein‐1 (HMGB1) is released from cells under various pathological conditions and it plays a pivotal role as an alarmin signaling tissue damage. Little is known about the impact of HMGB1 in bone repair and remodeling. To this aim, we focused on HMGB1‐induced effects on the in vitro osteoblast model SaOS‐2. Cell proliferation was stimulated with a maximum at concentration of 2.5 nM, and such a dose also stimulated cell migration and scratch wound healing. We then characterized the modulatory effect of HMGB1 on bone biology, by using osteogenesis/mineralization assays, a PCR array, and the analysis of a series of osteogenic markers. We performed also a proteomic screening using SWATH‐MS on SaOS‐2 cell exposed to HMGB1 and we provide evidence for proteins modulated in HMGB1 exposed cells. Taken together, our data demonstrate that SaOS‐2 cell proliferation, migration, and osteogenic differentiation were increased by HMGB1. We, therefore, propose that HMGB1 could be a potent bone‐remodeling signal but the physiological meaning of this property remains to be more ascertained. J. Cell. Biochem. 117: 2559–2569, 2016. © 2016 Wiley Periodicals, Inc.