Premium
Real‐Time In Vivo Confocal Fluorescence Imaging of Prostate Cancer Bone‐Marrow Micrometastasis Development at the Cellular Level in Nude Mice
Author(s) -
Miwa Shinji,
Toneri Makoto,
Igarashi Kentaro,
Yano Shuya,
Kimura Hiroaki,
Hayashi Katsuhiro,
Yamamoto Norio,
Tsuchiya Hiroyuki,
Hoffman Robert M.
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25545
Subject(s) - micrometastasis , bone marrow , green fluorescent protein , pathology , in vivo , prostate cancer , cancer research , confocal , medicine , cancer , chemistry , biology , metastasis , geometry , mathematics , biochemistry , microbiology and biotechnology , gene
In the present report, we demonstrate in vivo fluorescence imaging of bone‐marrow micrometastasis of prostate cancer at the cellular level in nude mice. PC‐3 human prostate cancer cells labeled with green fluorescent protein (GFP) or red fluorescent protein (RFP) were injected into the left ventricle or intratibial bone marrow of nude mice. PC‐3‐GFP, as well as selected high metastatic variants of PC‐3‐GFP, PC‐3‐GFP‐BM6 or PC‐3‐RFP were visualized by real‐time fluorescence imaging, to traffic and grow in the bone marrow. Formation of bone marrow micrometastasis could be imaged at the single‐cell level in live mice, using confocal microscopy. The ability to track bone marrow micrometastasis in real time at the cellular level provides a visual target for evaluating new therapeutics for this recalcitrant disease. J. Cell. Biochem. 117: 2533–2537, 2016. © 2016 Wiley Periodicals, Inc.