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Expression of Antioxidant Molecules and Heat Shock Protein 27 in Thyroid Tumors
Author(s) -
Wang Shanshan,
Yang Shucui,
Vlantis Alexander C.,
Liu Shirley Y.W.,
Ng Enders K.W.,
Chan Amy B.W.,
Wu Juekun,
Du Jing,
Wei Wei,
Liu Xiaoling,
Liu Zhimin,
Ng Siu Kwan,
van Hasselt C. Andrew,
Tong Michael C.F.,
Chen George G.
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25539
Subject(s) - glutathione peroxidase , thyroid , endocrinology , chemistry , medicine , glutathione , oxidative stress , glutathione reductase , superoxide dismutase , biology , biochemistry , enzyme
Oxidative stress‐induced DNA damage is a known causing factor for many types of tumors, but information on the role of oxidants and antioxidants in thyroid tumors is limited. The aim of this study was to determine antioxidant levels in thyroid tumors. In this study, tumor and its matched non‐tumor thyroid tissue samples were obtained from 53 patients with thyroid tumors. The levels of manganese superoxide dismutase (MnSOD), thioredoxin reductase 2 (TXNRD2), glutathione (GSH), glutathione peroxidase (Gpx), catalase (CAT), and 27 kd heat‐shock protein (hsp27) were determined in both thyroid tissue samples and cultured thyroid cells by immunohistochemical staining and western blot. Hydrogen peroxide (H 2 O 2 ) was used to generate oxidant stress in the cell culture experiments. We found that the levels of MnSOD, TXNRD2, GSH, Gpx, and Hsp27 were increased in both malignant and benign tumors, while the level of CAT was decreased. To verify the results of the tissue study, we treated cultured thyroid cells with H 2 O 2 and found the same pattern of antioxidant changes. Hsp27 was also increased after H 2 O 2 treatment. The expression of hsp27 was upregulated by 8.24‐, 6.96‐, and 3.09‐fold in thyroid cancer, follicular adenoma, multinodular goiter, respectively. Collectively, our study demonstrated that the levels of hsp27 together with MnSOD, TXNRD2, GSH, and Gpx were significantly upregulated by H 2 O 2 in thyroid tumors. The increase of these antioxidants is observed in both malignant and benign tumors, particularly in the former. The upregulation of antioxidants is likely a protective mechanism of tumor cells to maintain their survival and growth. J. Cell. Biochem. 117: 2473–2481, 2016. © 2016 Wiley Periodicals, Inc.