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Glycosidases Interact Selectively With Mannose‐6‐Phosphate Receptors of Bull Spermatozoa
Author(s) -
Aguilera Andrea C.,
Boschin Verónica,
Carvelli Lorena,
Cavicchia Juan C.,
Sosa Miguel A.
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25538
Subject(s) - mannose , mannose 6 phosphate , receptor , mannose 6 phosphate receptor , biochemistry , phosphate , enzyme , chemistry , mannosidase , glucuronidase , glycoside hydrolase , mannose receptor , biology , lysosome , in vitro , growth factor , macrophage
Glycosidases may play a role in sperm maturation during epididymal transit. In this work, we describe the interaction of these enzymes with bull spermatozoa. We found that β‐galactosidase associated to spermatozoa can be released under low ionic strength conditions, whereas the interaction of N‐acetyl‐β‐D‐glucosaminidase and β‐glucuronidase with spermatozoa appeared to be stronger. On the other hand, α‐mannosidase and α‐fucosidase cannot be removed from the gametes. In addition, part of N‐acetyl‐β‐D‐glucosaminidase, β‐galactosidase, and β‐glucuronidase can also be released by mannose‐6‐phosphate. Taking into account these data, we explored the presence of cation‐independent‐ and cation‐dependent‐mannose‐6‐phosphate receptors in the spermatozoa and found that cation‐independent mannose‐6‐phosphate receptor is highly expressed in bull spermatozoa and cation‐dependent‐mannose‐6‐phosphate receptor is expressed at a lesser extent. In addition, by immunofluorescence, we observed that cation‐independent‐mannose‐6‐phosphate receptor is mostly located at the acrosomal zone, whereas cation‐dependent‐mannose‐6‐phosphate receptor presents a different distribution pattern on spermatozoa during the epididymal transit. N‐acetyl‐β‐D‐glucosaminidase and β‐glucuronidase isolated from epididymal fluid interacted mostly with cation‐independent‐mannose‐6‐phosphate receptor, while β‐galactosidase was recognized by both receptors. We concluded that glycosidases might play different roles in bull spermatozoa and that mannos‐6‐phosphate receptors may act as recruiters of some enzymes. J. Cell. Biochem. 117: 2464–2472, 2016. © 2016 Wiley Periodicals, Inc.