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Arctigenin Inhibits Adipogenesis by Inducing AMPK Activation and Reduces Weight Gain in High‐Fat Diet‐Induced Obese Mice
Author(s) -
Han YoHan,
Kee JiYe,
Park Jinbong,
Kim HyeLin,
Jeong MiYoung,
Kim DaeSeung,
Jeon YongDeok,
Jung Yunu,
Youn DongHyun,
Kang JongWook,
So HongSeob,
Park Raekil,
Lee JongHyun,
Shin Soyoung,
Kim SuJin,
Um JaeYoung,
Hong SeungHeon
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25509
Subject(s) - adipogenesis , ampk , weight gain , endocrinology , medicine , chemistry , microbiology and biotechnology , body weight , adipose tissue , biology , biochemistry , phosphorylation , protein kinase a
Although arctigenin (ARC) has been reported to have some pharmacological effects such as anti‐inflammation, anti‐cancer, and antioxidant, there have been no reports on the anti‐obesity effect of ARC. The aim of this study is to investigate whether ARC has an anti‐obesity effect and mediates the AMP‐activated protein kinase (AMPK) pathway. We investigated the anti‐adipogenic effect of ARC using 3T3‐L1 pre‐adipocytes and human adipose tissue‐derived mesenchymal stem cells (hAMSCs). In high‐fat diet (HFD)‐induced obese mice, whether ARC can inhibit weight gain was investigated. We found that ARC reduced weight gain, fat pad weight, and triglycerides in HFD‐induced obese mice. ARC also inhibited the expression of peroxisome proliferator‐activated receptor gamma (PPARγ) and CCAAT/enhancer‐binding protein alpha (C/EBPα) in in vitro and in vivo. Furthermore, ARC induced the AMPK activation resulting in down‐modulation of adipogenesis‐related factors including PPARγ, C/EBPα, fatty acid synthase, adipocyte fatty acid‐binding protein, and lipoprotein lipase. This study demonstrates that ARC can reduce key adipogenic factors by activating the AMPK in vitro and in vivo and suggests a therapeutic implication of ARC for obesity treatment. J. Cell. Biochem. 117: 2067–2077, 2016. © 2016 Wiley Periodicals, Inc.