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Macrophage Recruitment Contributes to Regeneration of Mechanosensory Hair Cells in the Zebrafish Lateral Line
Author(s) -
Carrillo Simón A.,
AnguitaSalinas Consuelo,
Peña Oscar A.,
Morales Rodrigo A.,
MuñozSánchez Salomé,
MuñozMontecinos Carlos,
ParedesZúñiga Susana,
Tapia Karina,
Allende Miguel L.
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25487
Subject(s) - regeneration (biology) , zebrafish , microbiology and biotechnology , biology , macrophage , inflammation , immune system , immunology , anatomy , in vitro , genetics , gene
In vertebrates, damage to mechanosensory hair cells elicits an inflammatory response, including rapid recruitment of macrophages and neutrophils. While hair cells in amniotes usually become permanently lost, they readily regenerate in lower vertebrates such as fish. Damage to hair cells of the fish lateral line is followed by inflammation and rapid regeneration; however the role of immune cells in this process remains unknown. Here, we show that recruited macrophages are required for normal regeneration of lateral line hair cells after copper damage. We found that genetic ablation or local ablation using clodronate liposomes of macrophages recruited to the site of injury, significantly delays hair cell regeneration. Neutrophils, on the other hand, are not needed for this process. We anticipate our results to be a starting point for a more detailed description of extrinsic signals important for regeneration of mechanosensory cells in vertebrates. J. Cell. Biochem. 117: 1880–1889, 2016. © 2016 Wiley Periodicals, Inc.