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Titanium With Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway
Author(s) -
M.S. CastroRaucci Larissa,
S. Francischini Marcelo,
N. Teixeira Lucas,
P. Ferraz Emanuela,
B. Lopes Helena,
T. de Oliveira Paulo,
Hassan Mohammad Q.,
Rosa Adalberto L.,
Beloti Marcio M.
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25469
Subject(s) - nanotopography , osteoblast , runx2 , bone morphogenetic protein 2 , chemistry , microbiology and biotechnology , bone morphogenetic protein , signal transduction , gene silencing , alkaline phosphatase , cellular differentiation , biology , biochemistry , gene , enzyme , in vitro
We aimed at evaluating the effect of titanium (Ti) with nanotopography (Nano) on the endogenous expression of BMP‐2 and BMP‐4 and the relevance of this process to the nanotopography‐induced osteoblast differentiation. MC3T3‐E1 cells were grown on Nano and machined (Machined) Ti surfaces and the endogenous BMP‐2/4 expression and the effect of BMP receptor BMPR1A silencing in both osteoblast differentiation and expression of genes related to TGF‐β/BMP signaling were evaluated. Nano supported higher BMP‐2 gene and protein expression and upregulated the osteoblast differentiation compared with Machined Ti surface. The BMPR1A silencing inhibited the osteogenic potential induced by Nano Ti surface as indicated by reduced alkaline phosphatase (ALP), osteocalcin and RUNX2 gene expression, RUNX2 protein expression and ALP activity. In addition, the expression of genes related to TGF‐β/BMP signaling was deeply affected by BMPR1A‐silenced cells grown on Nano Ti surface. In conclusion, we have demonstrated for the first time that nanotopography induces osteoblast differentiation, at least in part, by upregulating the endogenous production of BMP‐2 and modulating BMP signaling pathway. J. Cell. Biochem. 117: 1718–1726, 2016. © 2015 Wiley Periodicals, Inc.