z-logo
Premium
Placenta Mesenchymal Stem Cell Derived Exosomes Confer Plasticity on Fibroblasts
Author(s) -
Tooi Masayuki,
Komaki Motohiro,
Morioka Chikako,
Honda Izumi,
Iwasaki Kengo,
Yokoyama Naoki,
Ayame Hirohito,
Izumi Yuichi,
Morita Ikuo
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25459
Subject(s) - homeobox protein nanog , mesenchymal stem cell , alkaline phosphatase , paracrine signalling , microbiology and biotechnology , chemistry , lipid droplet , oil red o , adipocyte , embryonic stem cell , cellular differentiation , andrology , biology , adipogenesis , biochemistry , adipose tissue , induced pluripotent stem cell , gene , medicine , receptor , enzyme
Mesenchymal stem cell (MSC)‐conditioned medium (MSC‐CM) has been reported to enhance wound healing. Exosomes contain nucleic acids, proteins, and lipids, and function as an intercellular communication vehicle for mediating some paracrine effects. However, the function of MSC‐derived exosomes (MSC‐exo) remains elusive. In this study, we isolated human placenta MSC (PlaMSC)‐derived exosomes (PlaMSC‐exo) and examined their function in vitro. PlaMSCs were isolated from human term placenta using enzymatic digestion. PlaMSC‐exo were prepared from the conditioned medium of PlaMSC (PlaMSC‐CM) by ultracentrifugation. The expression of stemness‐related genes, such as OCT4 and NANOG, in normal adult human dermal fibroblasts (NHDF) after incubation with PlaMSC‐exo was measured by real‐time reverse transcriptase PCR analysis (real‐time PCR). The effect of PlaMSC‐exo on OCT4 transcription activity was assessed using Oct4‐EGFP reporter mice‐derived dermal fibroblasts. The stimulating effects of PlaMSC‐exo on osteoblastic and adipocyte‐differentiation of NHDF were evaluated by alkaline phosphatase (ALP), and Alizarin red S‐ and oil red O‐staining, respectively. The expression of osteoblast‐ and adipocyte‐related genes was also assessed by real‐time PCR. The treatment of NHDF with PlaMSC‐exo significantly upregulated OCT4 and NANOG mRNA expression. PlaMSC‐exo also enhanced OCT4 transcription. The NHDF treated with PlaMSC‐exo exhibited osteoblastic and adipocyte‐differentiation in osteogenic and adipogenic induction media. PlaMSC‐exo increase the expression of OCT4 and NANOG mRNA in fibroblasts. As a result, PlaMSC‐exo influence the differentiation competence of fibroblasts to both osteoblastic and adipocyte‐differentiation. It shows a new feature of MSCs and the possibility of clinical application of MSC‐exo. J. Cell. Biochem. 117: 1658–1670, 2016. © 2015 Wiley Periodicals, Inc.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here