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Sclerostin Enhances Adipocyte Differentiation in 3T3‐L1 Cells
Author(s) -
Ukita Mayumi,
Yamaguchi Taihiko,
Ohata Noboru,
Tamura Masato
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25432
Subject(s) - sclerostin , osteocyte , adipocyte , adipogenesis , wnt signaling pathway , endocrinology , medicine , osteoblast , downregulation and upregulation , microbiology and biotechnology , adipose tissue , chemistry , transfection , lipid metabolism , biology , signal transduction , gene , biochemistry , in vitro
Sclerostin, a secreted protein encoded by the Sost gene, is produced by osteocytes and is inhibited by osteoblast differentiation and bone formation. Recently, a functional association between bone and fat tissue has been suggested, and a correlation between circulating sclerostin levels and lipid metabolism has been reported in humans. However, the effects of sclerostin on adipogenesis remain unexplored. In the present study, we examined the role of sclerostin in regulating adipocyte differentiation using 3T3‐L1 preadipocytes. In these cells, sclerostin enhanced adipocyte‐specific gene expression and the accumulation of lipid deposits. Sclerostin also upregulated CCAAT/enhancer binding protein β expression but not cell proliferation and caspase‐3/7 activities. Sclerostin also attenuated canonical Wnt3a‐inhibited adipocyte differentiation. Recently, the transcriptional modulator TAZ has been involved in the canonical Wnt signaling pathway. Sclerostin reduced TAZ‐responsive transcriptional activity and TAZ‐responsive gene expression. Transfection of 3T3‐L1 cells with TAZ siRNA increased the lipid deposits and adipogenic gene expression. These results show that sclerostin upregulates adipocyte differentiation in 3T3‐L1 cells, suggesting a possible role for the osteocyte‐derived sclerostin as a regulator of fat metabolism and as a reciprocal regulator of bone and adipose tissues metabolism. J. Cell. Biochem. 117: 1419–1428, 2016. © 2015 Wiley Periodicals, Inc.