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Anti‐Inflammatory Effect of Ascochlorin in LPS‐Stimulated RAW 264.7 Macrophage Cells Is Accompanied With the Down‐Regulation of iNOS, COX‐2 and Proinflammatory Cytokines Through NF‐κB, ERK1/2, and p38 Signaling Pathway
Author(s) -
Lee SookHyun,
Kwak ChoongHwan,
Lee SungKyun,
Ha SunHyung,
Park Junyoung,
Chung TaeWook,
Ha KiTae,
Suh SukJong,
Chang YoungChae,
Chang Hyeun Wook,
Lee YoungChoon,
Kang BongSeok,
Magae Junji,
Kim CheorlHo
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25383
Subject(s) - nitric oxide synthase , proinflammatory cytokine , tumor necrosis factor alpha , lipopolysaccharide , macrophage , nitric oxide , nf κb , p38 mitogen activated protein kinases , chemistry , microbiology and biotechnology , kinase , inflammation , biology , signal transduction , protein kinase a , biochemistry , immunology , endocrinology , in vitro
A natural compound C 23 H 32 O 4 Cl, ascochlorin (ASC) isolated from an incomplete fungus, Ascochyta viciae has been known to have several biological activities as an antibiotic, antifungal, anti‐cancer, anti‐hypolipidemic, and anti‐hypertension agent. In this study, anti‐inflammatory activity has been investigated in lipopolysaccharide (LPS)‐induced murine macrophage RAW 264.7 cells, since ASC has not been observed on the inflammatory events. The present study has clearly shown that ASC (1–50 μM) significantly suppressed the production of nitric oxide (NO) and prostaglandin E 2 (PGE 2 ) and decreased the gene expression of inducible NO synthase (iNOS) and cyclooxygenase‐2 (COX‐2) in a dose‐dependent manner. Moreover, ASC inhibited the mRNA expression and the protein secretion of interleukin (IL)‐1β and IL‐6 but not tumor necrosis factor (TNF)‐α in LPS‐stimulated RAW 264.7 macrophage cells. In addition, ASC suppressed nuclear translocation and DNA binding affinity of nuclear factor‐κB (NF‐κB). Furthermore, ASC down‐regulated phospho‐extracellular signal‐regulated kinase 1/2 (p‐ERK1/2) and p‐p38. These results demonstrate that ASC exhibits anti‐inflammatory effects in RAW 264.7 macrophage cells. J. Cell. Biochem. 117: 978–987, 2016. © 2015 Wiley Periodicals, Inc.