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Freezing of Fresh Wharton's Jelly From Human Umbilical Cords Yields High Post‐Thaw Mesenchymal Stem Cell Numbers for Cell‐Based Therapies
Author(s) -
Fong ChuiYee,
Subramanian Arjunan,
Biswas Arijit,
Bongso Ariff
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25375
Subject(s) - mesenchymal stem cell , wharton's jelly , andrology , umbilical cord , cord lining , cd90 , chemistry , cell , explant culture , viability assay , stem cell , microbiology and biotechnology , biology , anatomy , cd34 , adult stem cell , in vitro , biochemistry , medicine , endothelial stem cell
Some cord blood banks freeze entire pieces of UC (mixed cord, MC) which after post‐thaw yields mixed heterogeneous populations of mesenchymal stem cells (MSCs) from all its microanatomical compartments. Freezing of such entire tissues results in sub‐optimal post‐thaw cell recovery because of poor cryoprotectant diffusion and intracellular ice‐formation, heat and water transport issues, and damage to intercellular junctions. To develop a simple method of harvesting pure homogeneous MSCs for cord blood banks, we compared the post‐thaw behavior of three groups of frozen UC tissues: (i) freshly harvested WJ without cell separation; (ii) MSCs isolated from WJ (WJSC); and (iii) MC, WJ, and WJSC produced high post‐thaw cell survival rates (93.52 ± 6.12% to 90.83 ± 4.51%) and epithelioid monolayers within 24 h in primary culture whereas post‐thaw MC explants showed slow growth with mixed epithelioid and fibroblastic cell outgrowths after several days. Viability and proliferation rates of post‐thawed WJ and hWJSC were significantly greater than MC. Post‐thaw WJ and WJSC produced significantly greater CD24 + and CD108 + fluorescence intensities and significantly lower CD40 + contaminants. Post‐thaw WJ and WJSC produced significantly lesser annexin‐V‐positive and sub‐G1 cells and greater degrees of osteogenic and chondrogenic differentiation compared to MC. qRT‐PCR analysis of post‐thaw MC showed significant decreases in anti‐apoptotic gene expression (SURVIVIN, BCL2) and increases in pro‐apoptotic (BAX) and cell cycle regulator genes (P53, P21, ROCK 1) compared to WJ and WJSC. We conclude that freezing of fresh WJ is a simple and reliable method of generating large numbers of clinically utilizable MSCs for cell‐based therapies. J. Cell. Biochem. 117: 815–827, 2016. © 2015 Wiley Periodicals, Inc.