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PRDM1, a Tumor‐Suppressor Gene, is Induced by Genkwadaphnin in Human Colon Cancer SW620 Cells
Author(s) -
Kang HoBum,
Lee HaReum,
Jee Da Jung,
Shin SuHyun,
Nah SeongSu,
Yoon Sun Young,
Kim Jae Wha
Publication year - 2016
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25262
Subject(s) - suppressor , repressor , gene knockdown , cancer research , colorectal cancer , cell culture , cell growth , biology , microbiology and biotechnology , cancer , chemistry , gene , gene expression , genetics
ABSTRACT Genkwadaphnin (GD‐1) is isolated from the flower buds of Daphne genkwa Siebold et Zuccarini (Thymelaeaceae), and it has been used as a traditional Korean and Chinese medicine. In this study, the authors observe that GD‐1 inhibits the growth of the colon cancer cell line, SW620, through the up‐regulation of p21 expression in a PRDM1‐dependent manner. After treatment with GD‐1, the transcriptional repressor PRDM1 is prominently induced in SW620 cells. Furthermore, GD‐1 induce the phosphorylation of PKD1 and MEK and subsequently provide PRDM1 enhancement, resulting in the suppression of c‐Myc expression and the up‐regulation of p21. PKD1 knockdown using siRNA abrogates PRDM1 expression by GD‐1 and subsequently disrupts the regulation of c‐Myc and p21 expression. Treating SW620 cells with GD‐1 inhibits cell‐cycle progression and is characterized by the down‐regulation of c‐Myc followed by the up‐regulation of p21 expression. The up‐regulation of p21 by GD‐1 induces the growth arrest of the SW620 colon cancer cell line. Based on these data, the authors propose that GD‐1 has tumor‐suppressor activity that may contribute to the anti‐tumor effects of PRDM1 in colon cancer. J. Cell. Biochem. 117: 172–179, 2016. © 2015 Wiley Periodicals, Inc.