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SMAD3 and SP1/SP3 Transcription Factors Collaborate to Regulate Connective Tissue Growth Factor Gene Expression in Myoblasts in Response to Transforming Growth Factor β
Author(s) -
Córdova Gonzalo,
Rochard Alice,
RiquelmeGuzmán Camilo,
Cofré Catalina,
Scherman Daniel,
Bigey Pascal,
Brandan Enrique
Publication year - 2015
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25143
Subject(s) - ctgf , transforming growth factor , growth factor , biology , connective tissue , transcription factor , smad , myogenin , extracellular matrix , c2c12 , microbiology and biotechnology , sp1 transcription factor , gene expression , cancer research , myocyte , gene , myogenesis , genetics , promoter , receptor
Fibrotic disorders are characterized by an increase in extracellular matrix protein expression and deposition, Duchene Muscular Dystrophy being one of them. Among the factors that induce fibrosis are Transforming Growth Factor type β (TGF‐β) and the matricellular protein Connective Tissue Growth Factor (CTGF/CCN2), the latter being a target of the TGF‐β/SMAD signaling pathway and is the responsible for the profibrotic effects of TGF‐β. Both CTGF and TGF are increased in tissues affected by fibrosis but little is known about the regulation of the expression of CTGF mediated by TGF‐β in muscle cells. By using luciferase reporter assays, site directed mutagenesis and specific inhibitors in C2C12 cells; we described a novel SMAD Binding Element (SBE) located in the 5′ UTR region of the CTGF gene important for the TGF‐β‐mediated expression of CTGF in myoblasts. In addition, our results suggest that additional transcription factor binding sites (TFBS) present in the 5' UTR of the CTGF gene are important for this expression and that SP1/SP3 factors are involved in TGF‐β‐mediated CTGF expression. J. Cell. Biochem. 116: 1880–1887, 2015. © 2015 Wiley Periodicals, Inc.