Premium
NS5ATP9 Promotes Beclin 1‐Dependent Starvation‐Induced Autophagy of Hepatoblastoma Cells
Author(s) -
Quan Min,
Liu Shunai,
Wang Qi,
Li Guoli,
Zhang Yu,
Feng Shenghu,
Liang Jinqiu,
Cheng Jun
Publication year - 2015
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25111
Subject(s) - autophagy , gene silencing , microbiology and biotechnology , starvation , hepatoblastoma , biology , cell , cell growth , gene , apoptosis , genetics , medicine , endocrinology , radiology
NS5ATP9, a gene up‐regulated by NS5A, plays a crucial oncogenic role in several types of human tumours. However, the underlying mechanisms remain unclear. Autophagy, an evolutionarily conserved catabolic process, maintains cellular homeostasis under stress conditions, such as starvation, and plays a crucial role in tumour initiation and progression. Here, we report that NS5ATP9 mRNA and protein expression was up‐regulated in starved HepG2 cells and that the up‐regulated NS5ATP9 played a functional role in starvation‐induced autophagy. Overexpression or silencing of this gene showed contrasting effects on Beclin 1 and on starvation‐induced autophagy. Furthermore, NS5ATP9‐mediated autophagy is required for promotion of tumour cell growth, and this effect could be inhibited with 3‐methyladenine, chloroquine or by Beclin 1‐silencing. Thus, the mechanism for NS5ATP9‐promoted autophagy is Beclin 1‐dependent in the condition of starvation, and for hepatoblastoma cell growth is also Beclin 1‐dependent. J. Cell. Biochem. 116: 1574–1582, 2015. © 2015 Wiley Periodicals, Inc.