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BHBA Influences Bovine Hepatic Lipid Metabolism via AMPK Signaling Pathway
Author(s) -
Deng Qinghua,
Liu Guowen,
Liu Lei,
Zhang Yuming,
Yin Liheng,
Shi Xiaoxia,
Wang Jianguo,
Yuan Xue,
Sun Guoquan,
Li Yu,
Yang Wei,
Guo Lihui,
Zhang Renhe,
Wang Zhe,
Li Xiaobing,
Li Xinwei
Publication year - 2015
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25062
Subject(s) - ampk , signal transduction , lipid metabolism , sterol regulatory element binding protein , chemistry , amp activated protein kinase , peroxisome , protein kinase a , peroxisome proliferator activated receptor , microbiology and biotechnology , receptor , biochemistry , medicine , sterol , phosphorylation , biology , cholesterol
β‐hydroxybutyric acid (BHBA), an important metabolite in β‐oxidation, is involved in the development of ketosis in dairy cows. It is known that AMP‐activated protein kinase (AMPK) signaling pathway plays an important role in the regulation of lipid metabolism in hepatocytes. In the present study, bovine hepatocytes were treated with BHBA at variable concontrations and Compound C (Cpd C, an AMPK inhibitor) to investigate the effects of BHBA on the AMPK signaling pathway. The results showed that when the concentration of BHBA reached 1.2 mM, the AMPK signaling pathway was activated and the expression of sterol regulatory element binding protein‐1c (SREBP‐1c) as well as its target genes were significantly decreased. And these decreases were blocked by Cpd C. The binding activity and nucleus translocation of SREBP‐1c showed a similar trend. The expression of peroxisome proliferator activated receptor‐α (PPARα), carbohydrates response element binding protein (ChREBP) and their target genes were significantly increased while they were negatively suppressed by the Cpd C. The content of triglyceride (TG) had no obviously change in the BHBA and Cpd C‐treated groups. These results indicate that BHBA can activate AMPK signaling pathway and regulate lipid synthesis and lipid oxidation genes of AMPK but showed no effect on TG in bovine hepatocytes. J. Cell. Biochem. 116: 1070–1079, 2015. © 2015 Wiley Periodicals, Inc.