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Retracted : MiR‐17–5p Up‐Regulates YES1 to Modulate the Cell Cycle Progression and Apoptosis in Ovarian Cancer Cell Lines
Author(s) -
Li Lan,
He Li,
Zhao JianLi,
Xiao Jing,
Liu Min,
Li Xin,
Tang Hua
Publication year - 2015
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.25060
Subject(s) - cancer research , gene knockdown , ectopic expression , microrna , cell cycle , cell growth , biology , apoptosis , ovarian cancer , cell , cancer , cell culture , gene , genetics
MicroRNAs (miRNAs) are small, non‐coding RNAs that participate in the regulation of gene expression. Although many studies have demonstrated the involvement of miR‐17–5p in different cancers, little is known to its function in ovarian cancer. In this study, we demonstrated that overexpression of miR‐17–5p was able to enhance cell proliferation by promoting G1/S transition of the cell cycle and suppressing apoptosis in ES‐2 and OVCAR3 cell lines, whereas inhibition of miR‐17–5p yielded the reverse phenotype. YES1 was identified as a novel target gene of miR‐17–5p. Moreover, miR‐17–5p was found to directly bind to the 3′UTR of YES1 mRNA and up‐regulated its expression. Furthermore, knockdown of YES1 led to the suppression of proliferation and induced cell cycle arrest in ES‐2 and OVCAR3 cells. Ectopic expression of YES1 was able to reverse the effects of miR‐17–5p inhibition. Collectively, our results indicated that miR‐17–5p might play a role in human ovarian cancer by up‐regulating YES1 expression. J. Cell. Biochem. 116: 1050–1059, 2015. © 2015 Wiley Periodicals, Inc.