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miR‐372 Regulates Glioma Cell Proliferation and Invasion by Directly Targeting PHLPP2
Author(s) -
Chen Xin,
Hao Bin,
Liu Ying,
Dai Dongwei,
Han Guosheng,
Li Yanan,
Wu Xi,
Zhou Xiaoping,
Yue Zhijian,
Wang Laixing,
Cao Yiqun,
Liu Jianmin
Publication year - 2015
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24949
Subject(s) - glioma , downregulation and upregulation , gene knockdown , cancer research , microrna , gene silencing , pi3k/akt/mtor pathway , protein kinase b , carcinogenesis , cell growth , three prime untranslated region , biology , apoptosis , untranslated region , signal transduction , microbiology and biotechnology , messenger rna , cancer , gene , biochemistry , genetics
MicroRNAs are known to be involved in carcinogenesis and tumor progression in glioma. Recently, microRNA‐372 (miR‐372) has been proved to play a substantial role in several human cancers, but its functions in glioma remain unclear. In this study, we confirmed that miR‐372 was commonly upregulated in glioma cell lines and tissues. Downregulation of miR‐372 markedly inhibited cell proliferation and invasion and induced G1/S arrest and apoptosis. Consistently, the xenograft mouse model also unveiled the suppressive effects of miR‐372 knockdown on tumor growth. Further studies revealed that miR‐372 modulated the expression of PHLPP2 by directly targeting its 3′‐untranslated region (3′‐UTR) and that miR‐372 expression was inversely correlated with PHLPP2 expression in glioma samples. Silencing of PHLPP2 could rescue the inhibitory effect of miR‐372 inhibitor. Moreover, miR‐372 knockdown suppressed the phosphorylation levels of the major components of PI3K/Akt pathway including Akt, mTOR, and P70S6K. Taken together, our results suggest that miR‐372 functions as an oncogenic miRNA through targeting PHLPP2 in glioma. J. Cell. Biochem. 116: 225–232, 2015. © 2014 Wiley Periodicals, Inc.