Premium
ROCK‐2 Is Associated With Focal Adhesion Maturation During Myoblast Migration
Author(s) -
Goetsch K.P.,
Snyman C.,
Myburgh K.H.,
Niesler C.U.
Publication year - 2014
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24784
Subject(s) - focal adhesion , extracellular matrix , c2c12 , microbiology and biotechnology , adhesion , cell migration , cell adhesion , chemistry , myocyte , extracellular , cell , biology , signal transduction , myogenesis , biochemistry , organic chemistry
ABSTRACT Satellite cell migration is critical for skeletal muscle growth and regeneration. Controlled cell migration is dependent on the formation of mature focal adhesions between the cell and the underlying extracellular matrix (ECM). These cell–ECM interactions trigger the activation of signalling events such as the Rho/ROCK pathway. We have previously identified a specific role for ROCK‐2 during myoblast migration. In this study we report that ROCK inhibition with Y‐27632 increases C2C12 myoblast velocity, but at the expense of directional migration. In response to Y‐27632 an increased number of smaller focal adhesions were distributed across adhesion sites that in turn were clearly larger than sites in untreated cells, suggesting a reduction in focal adhesion maturation. We also confirm ROCK‐2 localisation to the focal adhesion sites in migrating myoblasts and demonstrate a change in the distribution of these ROCK‐2 containing adhesions in response to Y‐27632. Taken together, our observations provide further proof that ROCK‐2 regulates directional myoblast migration through focal adhesion formation and maturation. J. Cell. Biochem. 115: 1299–1307, 2014. © 2014 Wiley Periodicals, Inc.