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miR‐20a Promotes Prostate Cancer Invasion and Migration Through Targeting ABL2
Author(s) -
Qiang XiaoFei,
Zhang ZhengWei,
Liu Qian,
Sun Nan,
Pan LiangLiang,
Shen Jing,
Li Tong,
Yun Chen,
Li Hui,
Shi LiHua
Publication year - 2014
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24778
Subject(s) - du145 , prostate cancer , cancer research , gene knockdown , microrna , cell migration , tyrosine kinase , receptor tyrosine kinase , cancer , biology , cell , phosphorylation , medicine , receptor , cell culture , microbiology and biotechnology , lncap , gene , biochemistry , genetics
The aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. The present study found miR‐20a was significantly up‐regulated in prostate cancer compared with normal prostate tissues. Patients with a higher miR‐20a expression had a Gleason score of 7–10 and shorter survival time. The transwell and wound healing assays revealed that blocking expression of miR‐20a by miR‐20a ASO suppresses the invasion and migration of PC‐3 and DU145 cells in vitro and also inhibits tumor growth in vivo. Furthermore, we identified miR‐20a directly targets the ABL family non‐receptor tyrosine kinases ABL2 and negatively regulates the phosphorylation of its downstream gene p190RhoGAP. Knockdown of ABL2 promoted cell invasion and migration and we identified miR‐20a‐induced cell invasion and migration can be rescued by ABL2. In conclusion, our findings show that miR‐20a significantly contributes to the progression of prostate cancer by targeting ABL2. J. Cell. Biochem. 115: 1269–1276, 2014. © 2014 Wiley Periodicals, Inc.