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LIF/STAT3/SOCS3 Signaling Pathway in Murine Bone Marrow Stromal Cells Suppresses Osteoblast Differentiation
Author(s) -
Matsushita Kenta,
Itoh Shousaku,
Ikeda Shun,
Yamamoto Yumiko,
Yamauchi Yukako,
Hayashi Mikako
Publication year - 2014
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24777
Subject(s) - leukemia inhibitory factor , socs3 , osteoblast , stat protein , stat3 , microbiology and biotechnology , cytokine , stromal cell , chemistry , suppressor of cytokine signalling , signal transduction , bone marrow , gene knockdown , cancer research , biology , interleukin 6 , immunology , apoptosis , biochemistry , in vitro
ABSTRACT Leukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the interleukin‐6 family and is expressed by multiple tissue types. This study analyzed the effect of LIF on osteoblast differentiation using primary murine bone marrow stromal cells (BMSCs). Colony‐forming unit‐osteoblast formation by BMSCs was significantly suppressed by LIF treatment. To clarify the mechanism underlying the LIF suppressive effect on osteoblast differentiation, we analyzed the downstream signaling pathway of LIF. LIF/signal transducer and activator of transcription 3 (STAT3) signaling induces the expression of suppressor of cytokine signaling 3 (SOCS3). SOCS3 knockdown experiments have previously demonstrated that short‐hairpin SOCS3‐BMSCs reversed the LIF suppressive effect. Our results demonstrated that LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway. J. Cell. Biochem. 115: 1262–1268, 2014. © 2014 Wiley Periodicals, Inc.