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The secretome signature of colon cancer cell lines
Author(s) -
Imperlini Esther,
Colavita Irene,
Caterino Marianna,
Mirabelli Peppino,
Pagnozzi Daniela,
Vecchio Luigi Del,
Noto Rosa Di,
Ruoppolo Margherita,
Orrù Stefania
Publication year - 2013
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24600
Subject(s) - signature (topology) , colorectal cancer , computational biology , biology , cancer research , microbiology and biotechnology , cancer , genetics , mathematics , geometry
The definition of the secretome signature of a cancer cell line can be considered a potential tool to investigate tumor aggressiveness and a preclinical exploratory study required to optimize the search of cancer biomarkers. Dealing with a cell‐specific secretome limits the contamination by the major components of the human serum and reduces the range of dynamic concentrations among the secreted proteins, thus favouring under‐represented tissue‐specific species. The aim of the present study is to characterize the secretome of two human colon carcinoma cell lines, CaCo‐2 and HCT‐GEO, in order to evaluate differences and similarities of two colorectal cancer model systems. In this study, we identified more than 170 protein species, 64 more expressed in the secretome of CaCo‐2 cells and 54 more expressed in the secretome of HCT‐GEO cells; 58 proteins were shared by the two systems. Among them, more than 50% were deemed to be secretory according to their Gene Ontology annotation and/or to their SignalP or SecretomeP scores. Such a characterization allowed corroborating the potential of a cell culture‐based model in order to describe the cell‐specific invasive properties and to provide a list of putative cancer biomarkers. J. Cell. Biochem. 114: 2577–2587, 2013. © 2013 Wiley Periodicals, Inc.