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Post‐translational modifications in activation and inhibition of oct‐1–DNA binding complex in H2B and other diverse gene regulation: Prediction of interplay sites
Author(s) -
Shakoori Abdul Rauf,
Hoessli Daniel C.
Publication year - 2013
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24382
Subject(s) - pou domain , promoter , biology , gene , transcription factor , rna polymerase ii , microbiology and biotechnology , histone , dna binding domain , histone octamer , regulation of gene expression , gene expression , genetics , nucleosome , homeobox
Abstract Octamer DNA binding transcription factors play important roles in housekeeping and specific gene regulations. Octamer DNA binding transcription factor‐1 (Oct‐1), expressed ubiquitously, is a multifunctional molecule. The binding sites of Oct‐1 are the promoters of H2B gene and the genes of snRNA, U2, U6, and 7SK, yet Oct‐1 has been described as constitutively expressed transcription factor regulating the expression of housekeeping genes. Diverse tissue‐specific genes regulations by Oct‐1 include genes for interleukins (IL) 2, 3, 5; the granulocyte‐macrophagal colony‐stimulating factor, immunoglobulins α, β, Ly9; the endocrine‐associated Pit‐1 gene; the genes for gonadoliberin, prolactin, the thyroid transcription factor, and thyrotropin. The most interesting aspect of the gene regulations of Oct‐1 includes both activation and inhibition of transcription. These opposite regulations of Oct‐1 have been described through presence/absence of a post‐translational modification (PTM) in its different domains. We propose a mechanism of interplay of different PTMs or presence/absence of PTMs in the different domains of Oct‐1. We also suggest that the absence of phosphorylation and acetylation in G1 and S phases of the cell cycle is associated with interplay of methylation and O ‐GlcNAc modification. This interplay of O ‐GlcNAc modification with the phosphorylation and methylation with acetylation in POU sub‐domain of Oct‐1 may facilitate the formation of Oct‐1–DNA complex, consequently activating H2B gene transcription. Whereas, in G2 and M phases these sites are occupied by phosphate resulting in inhibition of Oct‐1–DNA complex formation leading to the suppression of H2B gene transcription. J. Cell. Biochem. 114: 266–274, 2013. © 2012 Wiley Periodicals, Inc.