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The effect of rotative stress on CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts
Author(s) -
Qing Hong Zhang,
Meng Tao Liu,
Yi Zhou,
Wei Liu,
Ju Xiang Shen,
Li Liu
Publication year - 2013
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24372
Subject(s) - fas ligand , rankl , osteoclast , tartrate resistant acid phosphatase , microbiology and biotechnology , gene expression , receptor , gene , chemistry , biology , activator (genetics) , apoptosis , programmed cell death , biochemistry
This study was designed to explore the effects of rotative stress on carbonic anhydrase II (CAII), TNF receptor superfamily member 6 (FAS), FAS ligand (FASL), osteoclast‐associated receptor (OSCAR), and tartrate‐resistant acid phosphatase (TRAP) gene expression in osteoclasts. Osteoclasts were induced from RAW264.7 cells cultured in medium containing recombinant murine soluble receptor activator of NF‐Kβ ligand (sRANKL). The mRNA and protein expression of CAII, FAS, FASL, OSCAR, and TRAP genes in osteoclasts was detected by RT‐PCR and Western blot, respectively, after osteoclasts were loaded at various rotative stress strengths and times. No significant differences in mRNA and protein expression were observed between any of the control groups ( P > 0.05). Importantly, rotative stress had a significant effect on the mRNA and protein expression of these genes ( P < 0.05). We found a negative relationship between rotative stress strength and prolonged loading time and the expression of FAS/FASL genes in osteoclasts. In addition, there was a positive relationship between rotative stress strength and prolonged loading time and the expression of CAII, OSCAR, or TRAP genes in osteoclasts. Based on these results, rotative stress has a significant effect on CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts. J. Cell. Biochem. 114: 388–397, 2013. © 2012 Wiley Periodicals, Inc.