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FOXO3a/p27 kip1 expression and essential role after acute spinal cord injury in adult rat
Author(s) -
Zhang Shuangwei,
Huan Weipeng,
Wei Haixiang,
Shi Jinlong,
Fan Jinpeng,
Zhao Jian,
Shen Aiguo,
Teng Honglin
Publication year - 2013
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24371
Subject(s) - ly294002 , pi3k/akt/mtor pathway , microbiology and biotechnology , cell cycle , spinal cord injury , biology , western blot , neurogenesis , kinase , spinal cord , cell , signal transduction , neuroscience , biochemistry , genetics , gene
FOXO3a (Forkhead Class box O3a), as an important direct target of the phosphatidylinositol 3‐kinase (PI3K)/protein B (Akt) pathway, which regulates the cell survival and the cell‐cycle progression. Recent reports showed that FOXO3a could inhibit cell‐cycle progression at the G1/S transition by controlling transcription of the cyclin‐dependent kinase inhibitor p27 kip1 , which is also a key regulator of the mammalian neurogenesis. To elucidate the expression and role of FOXO3a in nervous system lesion and repair, we performed an acute spinal cord contusion injury (SCI) model in adult rats, which showed a temporal–spatial expression pattern of FOXO3a. Temporally, FOXO3a protein level significantly reduced day 3 after injury, and following FOXO3a down‐regulation, p27 kip1 protein and mRNA levels were also decreased after injury. Spatially, decreased levels of FOXO3a and p27 kip1 were predominant in astrocytes, which were regenerating axons and largely proliferated after injury. Furthermore in vitro, Western blot analysis, RT‐PCR, and immunofluorescence staining analysis demonstrated the relationship between FOXO3a and p27 kip1 in primary astrocytes. FOXO3a modulated the cell cycle by transcriptional regulation of p27 kip1 in astrocytes. Administration of the PI3K pharmacological inhibitor LY294002 abrogated this effect by regulating FOXO3a and p27 kip1 expression and subcellular localization. These results suggest that decreased levels of FOXO3a and p27 kip1 in spinal cord are involved in axonal regeneration and the proliferation of glial cells after SCI. J. Cell. Biochem. 114: 354–365, 2013. © 2012 Wiley Periodicals, Inc.