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ATF5, a possible regulator of osteogenic differentiation in human adipose‐derived stem cells
Author(s) -
Leong David Tai,
Abraham Mohan Chothirakottu,
Gupta Anurag,
Lim ThiamChye,
Chew Fook Tim,
Hutmacher Dietmar Werner
Publication year - 2012
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24150
Subject(s) - stem cell , adipose tissue , transcriptome , microbiology and biotechnology , embryonic stem cell , biology , transcription factor , regulator , cellular differentiation , gene knockdown , cell culture , endocrinology , gene expression , genetics , gene
The regulatory pathways involved in maintaining the pluripotency of embryonic stem cells are partially known, whereas the regulatory pathways governing adult stem cells and their “stem‐ness” are characterized to an even lesser extent. We, therefore, screened the transcriptome profiles of 20 osteogenically induced adult human adipose‐derived stem cell (ADSC) populations and investigated for putative transcription factors that could regulate the osteogenic differentiation of these ADSC. We studied a subgroup of donors' samples that had a disparate osteogenic response transcriptome from that of induced human fetal osteoblasts and the rest of the induced human ADSC samples. From our statistical analysis, we found activating transcription factor 5 (ATF5) to be significantly and consistently down‐regulated in a randomized time‐course study of osteogenically differentiated adipose‐derived stem cells from human donor samples. Knockdown of ATF5 with siRNA showed an increased sensitivity to osteogenic induction. This evidence suggests a role for ATF5 in the regulation of osteogenic differentiation in adipose‐derived stem cells. To our knowledge, this is the first report that indicates a novel role of transcription factors in regulating osteogenic differentiation in adult or tissue specific stem cells. J. Cell. Biochem. 113: 2744–2753, 2012. © 2012 Wiley Periodicals, Inc.