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Mangiferin aglycone attenuates radiation‐induced damage on human intestinal epithelial cells
Author(s) -
Lei Jixiao,
Zhou Chuanfeng,
Hu Honggang,
Hu Lina,
Zhao Meijia,
Yang Yanyong,
Chuai Yunhai,
Ni Jin,
Cai Jianming
Publication year - 2012
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.24138
Subject(s) - mangiferin , aglycone , chemistry , ionizing radiation , pharmacology , dna damage , biochemistry , biology , dna , irradiation , stereochemistry , glycoside , chromatography , physics , nuclear physics
Recent studies suggest that mangiferin aglycone (norathyriol) has great potential as a novel radioprotector without any known toxic side effects. In this study, we assessed the protective effects of mangiferin aglycone against radiation‐induced injuries on normal human intestinal epithelial cells (HIECs), while using mangiferin as a reference compound. The in vitro experiments showed that pretreatment of either mangiferin aglycone or mangiferin could inhibit cytotoxic effects of ionizing irradiation (IR) on HIECs. Cellular changes were estimated by measuring cell viability, clonogenic surviving rate, and apoptotic rate. Compared to mangiferin, we found mangiferin aglycone had greater radioprotective effects of mangiferin aglycone on HIECs. It has been demonstrated that the cytotoxicity of ionizing radiation relates to its capacity to induce DNA damage. In view of this, we monitored DNA double‐strand breaks (DSBs) using γH2AX foci formation to test whether mangiferin aglycone and mangiferin could modulate genotoxic effects of radiation. It shows that mangiferin aglycone could eliminate 46.8% of the total DSBs of the cells exposed to 2 Gy IR, which is significantly better than mangiferin. Complementing earlier results from our group, it appears possible to conclude that mangiferin aglycone presents potential useful effects on IR‐induced damage and may be a better radioprotective agent than mangiferin therapeutically. J. Cell. Biochem. 113: 2633–2642, 2012. © 2012 Wiley Periodicals, Inc.