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GRP receptors are present in non small cell lung cancer cells
Author(s) -
Moody T. W.,
Zia F.,
Venugopal R.,
Fagarasan M.,
Oie H.,
Hu V.
Publication year - 1996
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240630520
Subject(s) - bombesin , receptor , clonogenic assay , cytosol , cell , cancer research , cell growth , biology , medicine , endocrinology , microbiology and biotechnology , chemistry , biochemistry , neuropeptide , enzyme
Previously, GRP receptors wer charachterized in sasmll cell lung cancer cells and here non‐small cell lung cancer (NSCLC) cells were investigated: ( 125 I‐Tyr 4 ) bombesin (BN) or 125 I‐GRP bound with high affinity to NCI‐H720 (lung carcioid) and NCI‐H1299 (large cell carcinoma) cells. Binding was specific, time dependent, and saturable. Specific ( 125 I‐Tyr 4 ) BN binding to NCI‐H1299 cells was inhibited with high affinity by GRP, BN, GRP 14–27 , (D‐Phe 6 )BN 6–13 methyl ester, moderate affinity by NMB, and low affinity by NMB, and low, and low affinity by GRP 1–16 . BN (10nM) transiently elevated cytosolic calcium in a dose dependent manner. BN caused translocation of protein kinase C from the cytosol to the membrane and the translocation caused by BN was reversed by (D‐Phe 6 )BN 6–13 methylester. BN stimulated arachidonic acid release and the increase caused by BN was reversed by (D‐Phe 6 )BN 6–13 methylester. Using a clonogenic assay, BN stimulated the growth of NCI‐H720 cells, and ythe number of colonies was reduced using (D‐Phe 6 )BN 6–13 methylester. These data suggest that GRP receptors that are present in lung carcinoid and NSCLC cells may regulate proliferation. © 1996 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.