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Bombesin receptor structure and expression in human lung carcinoma cell lines
Author(s) -
Fathi Zahra,
Way James W.,
Corjay Martha H.,
Viallet Jean,
Sausville Edward A.,
Battey James F.
Publication year - 1996
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240630519
Subject(s) - bombesin , gastrin releasing peptide , receptor , biology , g protein coupled receptor , autocrine signalling , gq alpha subunit , signal transduction , phospholipase c , endocrinology , microbiology and biotechnology , medicine , biochemistry , neuropeptide
Mammalian bombesin‐like peptides gastrin‐releasing peptide (GRP) and neuromedin B (NMB) are regulatory neuropeptides involved in numerous physiologic processes, and have been implicated as autocrine and/or paracrine growth factors in human lung carcinoma. Three structurally and pharmacologically distinct bombesin receptor subtypes have been isolated and characterized: the gastrin releasing peptide receptor (GRP‐R), the neuromedin B receptor (NMB‐R), and bombesin receptor subtype‐3 (BRS‐3). The three receptors are structurally related, sharing about 50% amino acid identity. They are members of the G‐protein coupled receptor superfamily with a seven predicted transmembrane segment topology charcteristic of receptors in this family. The signal transduction pathway for GRP‐R and NMB‐R involves coupling to a pertussis‐toxin insensitive G‐protien, activation of phospholipase C (PLC), generation of inositol trisphosphate (IP3), release of intracellular calcium, and activation of protein kinase C. While all three bombesin receptors are activated by bombesin agonists, GRP‐R, and NMB‐R, and BRS‐3 have very different affinities for the mammalian bombesin‐like peptides GRP and NMB, as well as bombesin receptor antagonists. The three bombesin receptor subtypes are expressed in an overlapping subset of human lung carcinoma cell lines. Any therapeutic strategy based on modulation of bombesin growth responses in human lung carcinoma cell lines. Any therapeutic strategy based on moducation of bombesin growth reponses in human lung carcinoma would be well served to take into account the pharmacologic heterogeneity of the relevant receptors. © 1996 Wiley‐Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.