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Citric acid extracts a specific set of proteins from isolated cell nuclei
Author(s) -
Rosenberger Uwe,
Shakibaei Mehdi,
Weise Christoph,
Franke Peter,
Buchner Klaus
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240590207
Subject(s) - citric acid , nucleoplasm , nuclear membrane , nuclear protein , cell nucleus , nuclear lamina , microbiology and biotechnology , rna , biochemistry , biology , cell membrane , membrane , cell cortex , inner membrane , cell , cytoskeleton , chemistry , nucleus , nucleolus , gene , transcription factor
The treatment of isolated cell nuclei with citric acid was described as a method for separating inner and outer nuclear membrane. Using cell nuclei from bovine cerebral cortex, we can show that citric acid does not cause a separation of the two nuclear membranes, but extracts a specific set of proteins from the nuclei. The extraction of proteins is not just an effect of damaging the nuclear membrane or destructing the cytoskeleton, but rather a specific effect of citric acid treatment. One of the extracted proteins, chosen as a marker for the putative outer nuclear membrane fraction, has an apparent molecular weight of 145 kDa and is located in the nucleoplasm as shown by immunofluorescence microscopy. By sequencing tryptic peptides it was identified as RNA helicase A, an abundant nuclear protein assumed to participate in the processing of mRNA. © 1995 Wiley‐Liss, Inc.