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Metabolism of 3 H‐1α,25‐dihydroxyvitamin D 3 in cultured human keratinocytes
Author(s) -
Ray Swapna,
Ray Rahul,
Holick Michael F.
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240590113
Subject(s) - incubation , metabolism , catabolism , calcitriol , cell culture , chemistry , biochemistry , keratinocyte , biology , vitamin d and neurology , endocrinology , in vitro , genetics
In the present investigation we studied the metabolism of 1α,25‐dihydroxy‐[1β‐ 3 H] vitamin D 3 ( 3 H‐1,25(OH) 2 D 3 ) in culture‐grown human keratinocytes (CHK). Our results showed that the cellular uptake of 3 H‐1,25(OH) 2 D 3 , upon incubation with CHK, occurred very rapidly; and it paralleled a decrease in the concentration of 3 H‐1,25(OH) 2 D 3 in the medium. The amount of 3 H‐calcitroic acid, on the other hand, increased slowly in the medium, while the concentration of 3 H‐calcitroic acid in the cell remained undetectable during the whole period of incubation. When the cells were preincubated with 1,25(OH) 2 D 3 (10 −8 M), conversion of 3 H‐1,25(OH) 2 D 3 to 3 H‐calcitroic acid increased almost twofold, indicating that 1,25(OH) 2 D 3 catalyzed its own catabolism. © 1995 Wiley‐Liss, Inc.