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Dysfunction of protein kinase F A /GSK‐3α in lymphocytes of patients with schizophrenic disorder
Author(s) -
Yang ShiawDer,
Yu JauSong,
Lee TsongTze,
Yang ChuanChing,
Ni MeiHui,
Yang YongYi
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240590112
Subject(s) - gsk 3 , protein kinase a , phosphatase , kinase , cyclin dependent kinase 9 , chemistry , mitogen activated protein kinase kinase , enzyme , biochemistry , microbiology and biotechnology , biology
As compared to normal people, the lymphocytes of patients with schizophrenia were found to have an impairment of ATP. Mg‐dependent protein phosphatase activation. More importantly, the impaired protein phosphatase activation in the lymphocytes of schizophrenic patients could be consistently and completely restored to normal by exogenous pure protein kinase F A /glycogen synthase kinase‐3α (kinase F A /GSK‐3α) (the activating factor of ATP.Mg‐dependent protein phosphatase), indicating that the molecular mechanism for the impaired protein phosphatase activation in schizophrenic patients may be due to a functional loss of kinase F A /GSK‐3α immunoblotting and kinase activity analysis in an anti‐kinase F A /GSK‐3α immunoprecipitate further demonstrate that both cellular activities and protein levels of kinase F A /GSK‐3α in the lymphocytes of schizophrenic patients were greatly impared as compared to normal controls. Statistical analysis revealed that the lymphocytes isolated from 37 normal people contain kinase F A /GSK‐3α activity in the high levels of 14.8 ± 2.4 units/mg of cell protein, whereas the lymphocytes of 48 patients with schizophrenic disorder contain kinase F A /GSK‐3α activity in the low levels of 2.8 ± 1.6 units/mg, indicating that the different levels of kinase F A /GSK‐3α activity between schizophrenic patients and normal people are statistically significant. Taken together, the results provide intial evidence that patients with schizophrenic disorder may have a common impairment in the protein levels and cellular activities of kinase F A /GSK‐3α, a multisubstrate protein kinase and a multisubstrate protein phosphatase activator in their lymphocytes. © 1995 Wiley‐Liss, Inc.