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β 3 ‐Adrenergic activation of adenylyl cyclase in mouse white adipocytes: modulation by GTP and effect of obesity
Author(s) -
BéginHeick Nicole
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240580409
Subject(s) - adenylyl cyclase , medicine , endocrinology , white adipose tissue , chemistry , lipolysis , gtp' , adrenergic , receptor , adrenergic receptor , brown adipose tissue , adipose tissue , biology , enzyme , biochemistry
Lipolysis and adenylyl cyclase (AC) activation in response to β‐adrenergic agents are abnormally low in white epididymal adipose tissue (WAT) of the ob/ob mouse. The abundance of G‐proteins (G s α and G i α) linked to AC is also abnormally low. By contrast, β‐adrenergic receptor (β‐AR) levels were previously found to be normal in WAT and elevated in liver. The relative importance of various forms of the β‐AR in mouse WAT was reassessed in view of the discovery of the β 3 ‐AR. The results show that (1) the β 3 ‐AR is mainly responsible for AC activation in lean‐mouse WAT; (2) the β 3 ‐AR is only partly responsible for AC activation in obese mouse WAT; and (3) GTP modulates β 3 —‐but not β 1 —‐or β 2 ‐AR activation of AC in a biphasic manner. Therefore, the β 3 ‐AR appears responsible for the well‐known bimodal effect of GTP on β‐adrenergic receptor‐mediated AC activity in WAT.