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POU‐domain gene expression in the gastrointestinal tract
Author(s) -
Fuller Peter J.,
Beveridge Dianne J.,
Taylor Russell G.
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240580214
Subject(s) - pou domain , gastrointestinal tract , biology , gene , ileum , gene expression , messenger rna , regulation of gene expression , microbiology and biotechnology , genetics , homeobox , endocrinology , biochemistry
Abstract The process of self‐renewal which occurs in the gastrointestinal epithelium is greatly amplified and accelerated during the intestinal adaptation which occurs in the residual ileum after massive small bowel resection (MSBR). As with growth and development, these processes must involve the coordinated regulation of many genes. Several families of nuclear proteins are known to be involved in the control of gene expression during development including the POU‐domain genes; their expression has not been characterized in the gastrointestinal tract during normal cellular renewal or adaptation, and POU‐domain encoding cDNAs were cloned from ileal RNA. Three known genes were cloned: Oct‐1, Brn‐1 and Tst‐1 but no novel members of this gene family were identified. The encoded sequence for rat Oct‐1 differs from that previously reported. Oct‐1 is relatively ubiquitously expressed with increased expression during both development and adaptation. Minimal expression of Tst‐1 was observed. Brn‐1 exhibits limited expression in the adult gastrointestinal tract. but may play a role in the fetal gastrointestinal tract.