Premium
Zeolite a inhibits osteoclast‐mediated bone resorption in vitro
Author(s) -
Schütze N.,
Oursler M. J.,
Nolan J.,
Riggs B. L.,
Spelsberg T. C.
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240580106
Subject(s) - osteoclast , zeolite , bone resorption , chemistry , cathepsin , resorption , cathepsin b , cathepsin k , diphosphonates , biochemistry , in vitro , enzyme , medicine , endocrinology , biology , catalysis
The effects of Zeolite A on Bone resorbing activity of highly purified avian osteoclasts were analyzed. The present study demonstrates that when 100 pg/ml of acid‐treated Zeolite A is added to the media the number of pits per osteoclast is reduced 3‐fold at 24h after treatment. Secreted cathepsin B enzyme activity was also reduced 3‐fold. A similar reduction in pit number per osteoclast was measured following 48 h of treatment with Zeolite A but there appeared to be less reduction of cathepsin B enzyme activity. The effects on pit number and cathepsin B Protein activity were Zeolit dose dependent. The structure of the compound seemed to be responsible for the effects measured since compounds used to represent constituents of Zeolite A (silicon dioxide and aluminum cholride) failed to inhibit bone resorption or reduce the level of secreted cathepsin B enzyme activity. Thus the molicular architecture of Zeolite A or a derivative there of appears to be inportant. In conclusion, the data indicate that Zeolite A can inhibit bone resorption. Together with previous data on osteoblasts, this might suggest a potential positive activity of intact Zeolite A or a partial substructure of Zeolite A on bone turnover. © Wiley‐Liss, Inc.