Premium
Sequence‐specific DNA binding activities of nuclear matrix proteins of mammalian lens epithelial cells
Author(s) -
Bagchi M.,
Van Wijnen A.,
Katar M.,
Merriman H.,
Lian J.,
Stein J.,
Stein G.,
Maisel H.
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240580102
Subject(s) - nuclear matrix , nuclear protein , matrix (chemical analysis) , microbiology and biotechnology , dna , biology , dna binding protein , lens (geology) , nuclear dna , chemistry , biochemistry , transcription factor , gene , paleontology , chromatography , chromatin , mitochondrial dna
This study examines matrix and nonmatrix nuclear proteins of the rabbit lens epithelial cells. The nuclear matrix proteins were isolated by modified Penman technique, which requires presence of detergents and nucleases, whereas nonmatrix nuclear proteins were obtained by high salt extraction. The data from these experiments revealed presence of DNA binding activities for SP‐1 and OCT‐1 proteins in both matrix and non‐matrix compartments of rabbit lens epithelial cells. Comparison of the relative abundance of SP‐1 and OCT‐1 binding activities in nuclear matrix and nonmatrix fraction suggest the distribution between these two compartment is cell type specific and possibly related to the control of cell growth. © Wiley‐Liss, Inc.