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Insulin receptor overexpression in 184B5 human mammary epithelial cells induces a ligand‐dependent transformed phenotype
Author(s) -
Frittitta Lucia,
Vigneri Riccardo,
Stampfer Martha R.,
Goldfine Ira D.
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240570411
Subject(s) - transfection , insulin receptor , receptor , irs2 , cell culture , insulin , receptor tyrosine kinase , cell , biology , endocrinology , microbiology and biotechnology , insulin receptor substrate , tyrosine kinase , medicine , chemistry , biochemistry , insulin resistance , genetics
To determine the role of the insulin receptor overexpression in breast epithelial cell transformation, the 184B5 human breast epithelial cell line was transfected with human insulin receptor cDNA. In two cell lines transfected with and overexpressing human insulin receptors (IR) (223.8 and 184.5 ng IR/10 6 cells), but not in untransfected cells, insulin binding and tyrosine kinase activity were elevated, and insulin induced a dose‐dependent increase in colony formation in soft agar.