Premium
Activation of the C‐fos promoter by increased internal pH
Author(s) -
Murguía José R.,
De Vries Luc,
GomezGarcía Lourdes,
Schönthal Axel,
Perona Rosario
Publication year - 1995
Publication title -
journal of cellular biochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.028
H-Index - 165
eISSN - 1097-4644
pISSN - 0730-2312
DOI - 10.1002/jcb.240570407
Subject(s) - transactivation , transfection , intracellular ph , transcription factor , intracellular , microbiology and biotechnology , chemistry , kinase , protein kinase a , biology , biochemistry , gene
Changes in intracellular pH (pHin) take part in the mitogenic response. Their importance has been stressed by the finding that mouse fibroblasts expressing a yeast proton pumping ATPase ( PMA1 ) exhibit a transformed phenotype and are tumorigenic. These cells do maintain a higher pHin, supporting the idea that elevated pHin may act as a proliferative trigger. Here we show that cells constitutively expressing PMA1 have higher levels of the AP‐1 transcription factor. The use of stable transfectants and transient transfection assays show that PMA1 activity induces transactivation of the c‐fos promoter. The activation of the promoter is mediated throughout the serum response element (SRE). The use of protein kinase C inhibitors suggests that AP‐1 activation is achieved through a pathway independent of protein kinase C.